Acti-stain™ 670 phalloidin from Cytoskeleton, Inc.

Product Specs
ItemActi-stain™ 670 phalloidin
CompanyCytoskeleton, Inc.
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberPHDN1-A
Quantity1 x 500 µl (300 slides)
Brand/TrademarkCytoskeleton, Inc

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Cytoskeleton, Inc.

1830 S. Acoma St.

Denver, Colorado 80223

United States

Phone: 303 322-2254
303 322-2257

Company Profile

Website: http://cytoskeleton.com/

(1) Src stimulates Abl-dependent phosphorylation of the guanine exchange factor Net1A to promote its cytosolic localization and cell motilityThe Journal of biological chemistryJuly 1, 2023Sprenger A, Carr HS, Ulu A, Frost JA.anti-rabbit-IgG conjugated to AlexaFluor 594 (Invitrogen). F-actin was visualized with Acti-Stain 670 Phalloidin (PHDN1-A; Cytoskeleton, Inc). Cells were then washed three times with PBST, rinsed with water, and mounted on

(2) Differential role for rapid proteostasis in Rho GTPase-mediated control of quiescent endothelial integrityJournal of Biological ChemistryApril 1, 2023Fabienne Podieh, Roos Wensveen, Max C. Overboom, Lotte Abbas, Jisca Majolée, Peter L. Hordijkwas stained with DAPI (#62248, Thermo Fisher Scientific) and F-actin with Acti-stain 670 phalloidin (#PHDN1-A, Cytoskeleton). In addition, propidium iodide (#P3566, Molecular Probes) and Hoechst Stain 33,342 (#sc-200908,

(3) A multiplex inhalation platform to model in situ like aerosol delivery in a breathing lung-on-chipFrontiers in PharmacologyMarch 6, 2023Arunima Sengupta, Aurélien Dorn, Mohammad Jamshidi, Magali Schwob, Widad Hassan, Lea Lara De Maddalena, Andreas Hugi, Andreas O. Stucki, Patrick Dorn, Thomas M. Marti, et al.actin cytoskeleton was visualized using the conjugated Alexa Fluor 647 phalloidin stain (1:300 dilution; C# PHDN1-A, Cytoskeleton, Inc.). Lastly, the stained membranes were sealed between two glass coverslips using a

(4) Myocardin‐related transcription factor's interaction with serum‐response factor is critical for outgrowth initiation, progression, and metastatic colonization of breast cancer cellsFASEB BioAdvancesAugust 1, 2022David Gau, Pooja Chawla, Ian Eder, Partha Royconjugated with either rhodamine (Life Technologies, catalog #R415) or far‐red (Cytoskeleton Inc, catalog #PHDN1‐A) fluorophore, for 20 min at room temperature. Stained cells were washed twice with PBS containing 0.02%

(5) A New Immortalized Human Alveolar Epithelial Cell Model to Study Lung Injury and Toxicity on a Breathing Lung-On-Chip SystemFront ToxicolJanuary 1, 2022Sengupta A, Roldan N, Kiener M, Froment L, Raggi G, Imler T, de Maddalena L, Rapet A, May T, Carius P, et al.actin cytoskeleton of the cells was visualized using the conjugated Alexa Fluor 647 phalloidin stain (C# PHDN1-A, Cytoskeleton, Inc.). Lastly, the stained membranes were sealed between two glass coverslips using mounting

(6) mito-TEMPO Attenuates Oxidative Stress and Mitochondrial Dysfunction in Noise-Induced Hearing Loss via Maintaining TFAM-mtDNA Interaction and Mitochondrial BiogenesisFrontiers in cellular neuroscienceJanuary 1, 2022Chen JW, Ma PW, Yuan H, Wang WL, Lu PH, Ding XR, Lun YQ, Yang Q, Lu LJ.rinsed with PBS three times, the specimens were incubated with Acti-Stain 670 Phalloidin (Cytoskeleton, #PHDN1-A, 1:70) or DAPI (Roche, #10236276001, 1 μg/ml) for 30 min at room temperature. For immunolabeling of

(7) KRAP tethers IP₃ receptors to actin and licenses them to evoke cytosolic Ca²⁺ signalsNature communicationsJuly 23, 2021Thillaiappan NB, Smith HA, Atakpa-Adaji P, Taylor CW.1,4,5-trisphosphate hexakis(propionoxymethyl) ester) was from SiChem (Bremen, Germany). Acti-stain 670 phalloidin (# PHDN1-A) was from Cytoskeleton (Denver, CO, USA). BAPTA (1,2-bis(o-aminophenoxy)ethane-N,N,N′,N′-tetraacetic

(8) Neuronal metabotropic glutamate receptor 8 protects against neurodegeneration in CNS inflammationThe Journal of experimental medicineMay 3, 2021Woo MS, Ufer F, Rothammer N, Di Liberto G, Binkle L, Haferkamp U, Sonner JK, Engler JB, Hornig S, Bauer S, et al.Cytoskeleton; catalog no. PHDH1-A) and actin-stain 670 phalloidin (1:100; Cytoskeleton; catalog no. PHDN1-A). To measure the influence of glutamate on human neuronal pCREB regulation, we pretreated hiPSC neuron

(9) Endothelial cells are not productively infected by SARS-CoV-2Clinical & translational immunologyJanuary 1, 2021Schimmel L, Chew KY, Stocks CJ, Yordanov TE, Essebier P, Kulasinghe A, Monkman J, Dos Santos Miggiolaro AFR, Cooper C, de Noronha L, et al.WB1:1000), Phalloidin‐Alexa555 (Cytoskeleton, PHDH1‐A, IF1:500), Phalloidin‐Alexa670 (Cytoskeleton, PHDN1‐A, IF1:200). Pre‐labelling of rabbit anti‐cleaved caspase 3 with Alexa Fluor 555 was performed using Zenon

(10) The interplay of Rac1 activity, ubiquitination and GDI binding and its consequences for endothelial cell spreadingPloS oneJanuary 1, 2021Majolée J, Podieh F, Hordijk PL, Kovačević I.was stained with DAPI (#62248, Thermo Fisher scientific) and F-actin with Acti-stain 670 phalloidin (#PHDN1-A, Cytoskeleton). The inhibitors PR619 (#S7130) and MLN7243 (#S8341) were used at 2.5 μM concentration.

(11) Fluorescent secreted bacterial effectors reveal active intravacuolar proliferation of Listeria monocytogenes in epithelial cellsPLOS PathogensOctober 12, 2020Caroline Peron-Cane, José-Carlos Fernandez, Julien Leblanc, Laure Wingertsmann, Arnaud Gautier, Nicolas Desprat, Alice Lebretonpromoter. At 1 and 3 h p.i., cells were fixed and stained with Acti-stain 670 phalloidin (Cytoskeleton #PHDN1-A, 70 nM) and DAPI as for immunofluorescence experiments. Fifteen to twenty fields per condition and time

(12) Ankyrin-B p.S646F undergoes increased proteasome degradation and reduces cell viability in the H9c2 rat ventricular cardiomyoblast cell lineBiochemistry and Cell BiologyApril 1, 2020Lena Chen, Catherine S.W. Choi, Juan C. Sanchez-Arias, Laura T. Arbour, Leigh Anne Swayne586-conjugated donkey anti-mouse IgG (1:600; A10037, Thermo-Fisher), in antibody buffer. Acti-stain 670 phalloidin (PHDN1-A, Cytoskeleton, Inc.) was used for coverslips that were not labelled with WGA. Qualitative determination

(13) NCAM regulates temporal specification of neural progenitor cells via profilin2 during corticogenesisThe Journal of cell biologyJanuary 6, 2020Huang R, Yuan DJ, Li S, Liang XS, Gao Y, Lan XY, Qin HM, Ma YF, Xu GY, Schachner M, et al.fluorophore 488, 555, or 647 were purchased from Invitrogen. Acti-stain 670 fluorescent phalloidin (PHDN1-A; Cytoskeleton) was used for F-actin staining. Alexa Fluor 594–conjugated DNase I (D12372; Molecular Probes

(14) Phosphorylation of Ci/Gli by Fused Family Kinases Promotes Hedgehog SignalingDevelopmental CellSeptember 1, 2019Yuhong Han, Bing Wang, Yong Suk Cho, Jian Zhu, Jiang Wu, Yongbin Chen, Jin JiangRecombinant Proteins" Leptomycin B,Millipore Sigma,Cat#L2913 Acti-Stain 670 phalloidin,Cytoskeleton,Cat#PHDN1-A Bluo-Gal,Thermo Fisher Scientific,Cat#15519028 L-Glutathione Reduced,Millipore Sigma,Cat#G4251 SAG,Millipore

(15) CSN5 inhibition triggers inflammatory signaling and Rho/ROCK-dependent loss of endothelial integrityScientific reportsMay 31, 2019Majolée J, Pronk MCA, Jim KK, van Bezu JSM, van der Sar AM, Hordijk PL, Kovačević I.antibody (anti-rabbit or anti-mouse 1:100 in 1% HSA/PBS), Acti-stain 670 phalloidin (Cytoskeleton) (#PHDN1-A) and DAPI (Thermo Fisher Scientific) for 1 hour at room temperature. Coverslips were mounted with Mowiol4-88/DABCO

(16) Ligand and Target Discovery by Fragment-Based Screening in Human CellsCellJanuary 26, 2017Parker CG, Galmozzi A, Wang Y, Correia BE, Sasaki K, Joslyn CM, Kim AS, Cavallaro CL, Lawrence RM, Johnson SR, et al.Reagent,LONZA,PT-7009 Hoechst 33342,Life Technologies,H1399 Acti-stain 670 phalloidin,Cytoskeleton,PHDN1-A TEV protease,"Backus et al., 2016",N/A Hemin,Fisher Scientific,ICN19882001 Protoporphyrin IX,Frontier

(17) BDNF/trkB Induction of Calcium Transients through Ca_v2.2 Calcium Channels in Motoneurons Corresponds to F-actin Assembly and Growth Cone Formation on β2-Chain Laminin (221)Frontiers in molecular neuroscienceJanuary 1, 2017Dombert B, Balk S, Lüningschrör P, Moradi M, Sivadasan R, Saal-Bauernschubert L, Jablonka S.phalloidin-Alexa546 (1:40, ThermoFisher Scientific, #A22283) and phalloidin-Acti stain 670 (1:50, Cytoskeleton, #PHDN1-A). Coverslips were embedded in mowiol (Sigma-Aldrich) and imaged with a confocal microscope. Specificity

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4/25/2023Excellent Acti-Stain 670 Phalloidin Matteo MassaroI used this product for staining of cytoskeleton on A549, MRC5 and SHSY5Y cells. I used a dilution factor of 1:25 for 30 minutes at room temperature. Used after 10 minutes of 0.1% Triton ... Read Review

Acti-stain™ 670 phalloidin from Cytoskeleton, Inc.

Cytoskeleton, Inc.

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Acti-stain™ 670 phalloidin from Cytoskeleton, Inc.

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